Multivariable Analysis and Phenotypic Diversity Studied for Some Barley Genotypes under Heat Stress
Keywords:
PCA, Hordeum vulgar, phenotypic diversity - heat stress index HI, heatmap analysisAbstract
Heat stress is one of most domineering abiotic stress influences that border barley production. Herein, three different field screening locations were carried out at Sakha, Mallawi and New-valley research stations, to identify the response of ten barley genotypes to different temperatures degrees using phenotypic diversity and, multivariable analysis during two consecutive seasons 2020/2021 and 2021/2022 under different temperatures degrees. Heat stress index (HI) activated a reduction in all traits ranged from lowest average reduction in plant height PH by (5.43 and 20.37%) to highest average reduction in no. of tillers /m2TM by (14.49 and 40.83 %) under Malawi (T2) and New Valley (T3) locations respectively as camper by Sakha, also high temperature enhancement all the genotypes to quicken flowering and days to maturity by average (7.24 and 8.35 %) under New Valley. Days to heading HD and to maturity MD exhibited a strong and significant negative relationship with all studied traits Loading principal component analysis PCA accounted 86.1% of the total variability, which PCA2 clarified 24.2 % of the total variability influenced by HD and MD which placed in the left side (negative). Scatter plot of PCA categorizing all the barley genotypes in four groups indicated that the Egyptian barley genotypes (Giza 137, Giza 138, line5, line 1 and line 3) were separate from the other genotypes and located in the right side with of PCA1 analysis cluster with a significant distance which could be considered as a heat tolerance genotypes. A cluster heatmap according their resulted form all studied traits showing that the ten barley genotypes were clustered into two main clusters, Giza 137, Giza 138, line5, line 1 and line 3 were the most closed genotypes together due to their tolerance to heat stress while line 2 and line 8 were the most closed genotypes together due to their sensitive to heat stress. Thus, we could use them as a source for future barley breeding programs for heat stress such as important step to get a new genotype with high heat tolerance and high yield.
References
M S Bartlett (1937) Properties of sufficiency and statistical tests. 160, 268.
L S Bates, R P Waldern, I D Teave (1973) Rapid determination of free proline for water stress studied. 39, 205-207.
M Bouslama, W T Schapaugh (1984) Stress tolerance in soybean. Part 1: Evaluation of three screening techniques for heat and drought tolerance. 24, 933-937.
M M Bradford (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilization the principle of protein-dye binding. 72, 248-254.
S Devi, Y Kumar, S Shehrawat (2021) Identification of heat tolerant barley genotypes based on heat susceptibility index. 13(2), 197-204.
H Elbasiouny, M Abowaly, A Gad, A Abu_Alkheir, F Elbehiry (2017) Restoration and sequestration of carbon and nitrogen in the degraded northern coastal area in Nile Delta, Egypt for climate change mitigation. 105–114.
Md Habib, Md Mannan, Md Karim, Md Miah, H Singh (2021) Water deficit stress tolerance assessment in barley cultivars using drought tolerance indices.
M Habouh, H Abo-Sapra (2025) Impact of Late Sowing on Performance of Barley Genotypes Under Aswan Conditions. 56(1), 33-47.
Ádám Horváth, Zita Berki, Krisztina Balla, Judit Bányai, Marianna Mayer, András Cseh, Tibor Kiss, Ildikó Karsai (2024) Field versus controlled environmental experiments to evaluate the heat stress response of barley (Hordeum vulgare L.). 228.
J Kaseva, K Hakala, M Högnäsbacka, L Jauhiainen, S J Himanen, R P Rötter, H Kahiluoto (2023) Assessing climate resilience of barley cultivars in northern conditions during 1980–2020. 293, 108856.
J Kim, R Savin, G A Slafer (2024) Quantifying pre-and post-anthesis heat waves on grain number and grain weight of contrasting wheat cultivars. 307, 109264.
ogender Kumar, Suman Devi, Divya Phougat, Harsh Chaurasia (2025) Estimation of stress tolerance indices for identification of heat tolerant genotypes in barley. 154, 890-902.
Mariey Samah, I Khatab, A M Shahein, E S A Abo-Marzoka, T Noreldin, A F Badawy, A A El-Naggar (2022) Comprehensive Selection Criteria for Some Barley Genotypes under Different Water Stress Treatments. 34(23), 1777-1791.
Mariey Samah, Mohamed Eman, Ghareeb Zeinab (2021) Genetic Diversity of Egyptian Barley using Agro-physiological Traits, grain quality and molecular markers. 10, 58-71.
Mariey Samah, El-Bialy MA, Khedr RA, Mohamed EN, Meleha AMI, Khatab IA (2023) Comprehensive evaluation and economic analysis in some barley genotypes under soil salinity. 7, 20-33.
Mariey Samah, Omnia Hashem, Anas Ahmed, Karima Ahmed, Hayam Elsawy (2023) Phenotypic and genotypic diversity analysis of some Egyptian barley cultivars (Hordeum vulgare L.) under different heat stress conditions. 101(2), 412-423.
T Metsalu, J Vilo (2015) ClustVis. 566.
A H Mohamed, A A Omar, A M Attya, M M A Elashtokhy, E M Zayed, R M Rizk (2021) Morphological and Molecular Characterization of Some Egyptian Six-Rowed Barley (Hordeum vulgare L.). 2527.
A Pour-Aboughadareh, A Barati, S A Koohkan, M Jabari, A Marzoghian, A Gholipoor, M Kheirgo (2022) Dissection of genotype-by-environment interaction and yield stability analysis in barley using AMMI model and stability statistics. 46(1), 19.
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