Polyphenol-Rich Collas-Coe a Study on Antioxidant Activity and Cellular Safety

Abstract

Polyphenols, known for their strong antioxidant properties, combat oxidative stress, enhance cell viability, and protect against free radical damage. These bioactive compounds hold immense potential for functional food and cosmetic innovations, supporting overall health and promoting skin resilience, offering exciting opportunities in nutraceutical and cosmetic product development. This study evaluates the bioactive properties and potential applications of Collas-Co�, a high-polyphenol ingredient with promising antioxidant activity. The total phenolic content (TPC) of Collas-Co� was determined to be 58.05 � 0.33 mg GAE/g. Its antioxidant capacity, assessed via ORAC assay, revealed a remarkably high scavenging activity of 15,221 � 409 �mol TE/100g. Furthermore, the DPPH assay demonstrated superior radical scavenging activity, with Collas-Co� achieving 85.67% inhibition at 2.5 mg/mL, outperforming gallic acid by approximately 15%. Cytotoxicity studies using primary human dermal fibroblasts indicated that Collas-Co� exhibited dose-dependent effects. At lower concentrations (0.39 mg/mL), it enhanced cell viability to 112.94%, suggesting potential protective or stimulatory properties. Moderate concentrations (0.78�6.25 mg/mL) showed tolerability, while higher concentrations (>12.50 mg/mL) resulted in cytotoxicity, with an IC50 of 50.50 � 2.07 mg/mL. These findings highlight Collas-Co� as a potent antioxidant with moderate cytotoxicity, suitable for functional foods and cosmetic applications.