Polyphenol-Rich Collas-Coe a Study on Antioxidant Activity and Cellular Safety
Keywords:
Digital radiography; Multiscale analysis; Pixel-level fusion; Image detail enhancement; Nondestructive testing, turbulence, computational fluid dynamics., Divergent Nozzle Grooving, Rocket nozzle, Flow field, Antioxidant, cytotoxicity, Polyphenol, CellularAbstract
Polyphenols, known for their strong antioxidant properties, combat oxidative stress, enhance cell viability, and protect against free radical damage. These bioactive compounds hold immense potential for functional food and cosmetic innovations, supporting overall health and promoting skin resilience, offering exciting opportunities in nutraceutical and cosmetic product development. This study evaluates the bioactive properties and potential applications of Collas-Co™, a high-polyphenol ingredient with promising antioxidant activity. The total phenolic content (TPC) of Collas-Co™ was determined to be 58.05 ± 0.33 mg GAE/g. Its antioxidant capacity, assessed via ORAC assay, revealed a remarkably high scavenging activity of 15,221 ± 409 µmol TE/100g. Furthermore, the DPPH assay demonstrated superior radical scavenging activity, with Collas-Co™ achieving 85.67% inhibition at 2.5 mg/mL, outperforming gallic acid by approximately 15%. Cytotoxicity studies using primary human dermal fibroblasts indicated that Collas-Co™ exhibited dose-dependent effects. At lower concentrations (0.39 mg/mL), it enhanced cell viability to 112.94%, suggesting potential protective or stimulatory properties. Moderate concentrations (0.78–6.25 mg/mL) showed tolerability, while higher concentrations (>12.50 mg/mL) resulted in cytotoxicity, with an IC50 of 50.50 ± 2.07 mg/mL. These findings highlight Collas-Co™ as a potent antioxidant with moderate cytotoxicity, suitable for functional foods and cosmetic applications.
References
I. Liguori, G. Russo, F. Curcio (2018) Oxidative stress, aging, and diseases. 13, 757-772.
K. B. Pandey, S. I. Rizvi (2009) Plant polyphenols as dietary antioxidants in human health and disease. 2(5), 270-278.
A. Scalbert, C. Manach, C. Morand (2005) Dietary polyphenols and the prevention of diseases. 45(4), 287-306.
R. L. Prior, X. Wu, K. Schaich (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. 53(10), 4290-4302.
M. Yamauchi, Y. Kitamura, H. Nagano (2024) DPPH measurements and structure-activity relationship studies on the antioxidant capacity of phenols. 13(3), 309.
M. D'Urso, N. A. Kurniawan (2020) Mechanical and physical regulation of fibroblast-myofibroblast transition: From cellular mechanoresponse to tissue pathology. 8, 609653.
V. L. Singleton, J. A. J. Rossi (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. 16, 144-158.
J. O. Kuti, H. B. Konuru (2004) Antioxidant capacity and phenolic content in leaf extracts of tree spinach (Cnidoscolus spp.). 52(1), 117-121.
N. B. Sadeer, D. Montesano, M. F. Mahomoodally (2020) The versatility of antioxidant assays in food science and safety—Chemistry, applications, strengths, and limitations. 9, 709.
A. Siddeega, N. M. AlKehayez, H. A. Abu-Hiamed (2021) Mode of action and determination of antioxidant activity in the dietary sources: An overview. 28, 1633-1644.
S. Kamiloglu, G. Sari, T. Ozdal, E. Capanoglu (2020) Guidelines for cell viability assays. 1(3), 332-349.
L. M. Yin, Y. Wei, Y. Wang (2013) Long term and standard incubations of WST-1 reagent reflect the same inhibitory trend of cell viability in rat airway smooth muscle cells. 10(1), 68-72.
M. D. S. Ribeiro, N. Sebastià, A. Montoro (2023) Strawberry (Fragaria× Ananassa) and Kiwifruit (Actinidia Deliciosa) extracts as potential radioprotective agents: Relation to their phytochemical composition and antioxidant capacity. 13(15), 8996.
L. Alnsour, R. Issa, S. Awwad (2022) Quantification of total phenols and antioxidants in coffee samples of different origins and evaluation of the effect of degree of roasting on their levels. 27(5), 1591.
J. R. B. Carvalho, A. N. Meireles, S. S. Marques (2023) Exploiting kinetic features of ORAC assay for evaluation of radical scavenging capacity. 12(2), 505.
S. Bhagwat, D. B. Haytowitz, J. M. Holden (2007) USDA database for the oxygen radical absorbance capacity (ORAC) of selected foods. 1-2.
F. Martinez-Morales, A. J. Alonso-Castro, J. R. Zapata-Morales (2020) Use of standardized units for a correct interpretation of IC50 values obtained from the inhibition of the DPPH radical by natural antioxidants. 74, 3325-3334.
M. Hadidi, R. Linan-Atero, M. Tarahi (2024) The potential health benefits of gallic acid: therapeutic and food applications. 13(8), 1001.
E. A. M. A. E. Mohamed, A. H. Mahran, S. H. E. Ashry (2023) The cytotoxic effect of cysteamine and its combinations with various endodontic intracanal medications on fibroblast cells: in vitro study. 47(1), 74.
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