New Markers of Coronary Heart Disease with Old Myocardial Infarction: Certain Variants of Relative Length of Telomeric Repeats

Abstract

One of the possible factors in the occurrence of coronary heart disease with myocardial infarction is oxidative stress.� Oxidative stress intensifies the formation of free radicals, causing defects in proteins and nucleic acids. This can lead� to their partial or complete destruction. For example, the result of such destruction of DNA molecules may be a� decrease in the length of telomeric repeats in the chromosomes of cells in coronary heart disease.�

Therefore, the aim of this work was to analyze the association of certain variants of the relative length of telomeric� repeats with coronary heart disease with old myocardial infarction (CHD with MI).�

To measure the length of telomeric repeats, whole blood was collected, with the following DNA isolation from nuclear� cells. The relative length of telomeric repeats was calculated based on the formula �2 to the power (-?Ct)�, where ?Ct� = Ct of telomeres-Ct of albumin. In this case, Ct of telomeres is the threshold cycle of the telomeric repeat, and Ct of� albumin is the threshold cycle of the albumin gene. The results of the relative length of telomeric repeats are presented� as a percentage of the calibrator. DNA isolated from HeLa cell line was used as a calibrator.�

By measuring variants of the relative length of telomeric repeats in the studied samples, an association of coronary� heart disease with myocardial infarction with VRLTR-46, VRLTR-49, VRLTR-51, VRLTR-53 and VRLTR-56 was� detected. At the same time, 6 of 21 variants of the relative length of telomeric repeats (VRLTR-63, VRLTR-65,� VRLTR-68, VRLTR-70, VRLTR-71 and VRLTR-73) had a protective effect in coronary heart disease with myocardial� infarction.�

Therefore, in the present research work, we propose new markers of coronary heart disease with old myocardial� infarction: certain variants of relative length of telomeric repeats (VRLTR).�